Journal: Cell reports

Article Title: Integrating Gene and Protein Expression Reveals Perturbed Functional Networks in Alzheimer’s Disease

doi: 10.1016/j.celrep.2019.06.073

Figure Lengend Snippet:

Article Snippet: Normal Horse Serum , Vector Laboratories , Cat# S-2000; RRID: AB_2336617.

Techniques: Plasmid Preparation, Recombinant, Software, Microscopy

Journal: The Journal of Biological Chemistry

Article Title: Metallothionein-I/II Promotes Axonal Regeneration in the Central Nervous System *

doi: 10.1074/jbc.M114.630574

Figure Lengend Snippet: Intravitreal injection of MT-I/II-injected promotes optic nerve regeneration in vivo. Fischer rats with optic nerve injury were injected intravitreally with 5 μl of normal saline (A), MT-I/II (5 μg/μl) (B), or had MT-I/II-soaked GF (C) placed over the injured retinal axons immediately after crushing the optic nerve; animals A–C had no signs of lens injury. With LI these rats were injected intravitreally with 5 μl of normal saline (D), MT-I/II (5 μg/μl) (E), and a close-up view of the axons with either saline (F) or MT-I/II (G) injection ∼1.25 mm from the lesion center. Regenerating axons were evaluated 2 weeks later by staining for GAP-43. Quantification of axonal density in regions 0–500, 500–1000, 1000–1500, and 1500–2000 μm was distal to the lesion site. Graph depicts average axonal density (square micrometer) ± S.E. for animals with no LI (H) and for animals with LI (*, p < 0.05; **, p < 0.01) (I). Scale bar, 100 μm. Fischer rats received intravitreal injection with 5 μl of MT-I/II (5 μg/μl) immediately after crushing the optic nerve, and there was no signs of lens injury (J). To assess regenerating axons, we labeled with CTB coupled to Alexa-488 intravitreally injected 3 days prior to sacrifice. The whole optic nerve was chemically cleared, and we visualized CTB-labeled fibers using a multiphoton microscope scanning 250 μm of the optic nerve. Scale bar, 100 μm. The white box in J is an area we reimaged at higher magnification to show the extent of CTB-labeled regenerating fibers (K). The white arrow is pointing to what appears to be a growth cone. scale bar, 25 μm.

Article Snippet: Spinal cord sections were immunostained using goat anti-CTB antibody (1:2000; List Biological), biotinylated donkey anti-goat IgG (1:200; List Biological), and avidin-biotin complex (Vector Laboratories).

Techniques: Injection, In Vivo, Staining, Labeling, Microscopy